Thursday, November 20, 2014

Problem-solving test: Pyrosequencing

Pyrosequencing I stumbled across this paper today. A very useful resource I'm sure many people could use:

Szeberenyi, J. (2013) Problem‐solving test: Pyrosequencing. Biochemistry and Molecular Biology Education, 41(2): 112-115.
Terms to be familiar with before you start to solve the test: Maxam–Gilbert sequencing, Sanger sequencing, gel electrophoresis, DNA synthesis reaction, polymerase chain reaction, template, primer, DNA polymerase, deoxyribonucleoside triphosphates, orthophosphate, pyrophosphate, nucleoside monophosphates, luminescence, acid anhydride bond, phosphodiester bond, mismatch, proofreading, 5′->3′ exonuclease.
Study the principle of pyrosequencing and answer the following multiple-choice questions.


  1. Replies
    1. You'll need to read the paper :-)

    2. Yes, I can find all the questions in the paper. Mainly, I'm focusing on DNA synthesis reaction.